Amplification:Article Title: Immunoengineering can overcome the glycocalyx armour of cancer cells.
Article Snippet: Cancer cell glycocalyx is a major line of defence against immune surveillance.. However, how specific physical properties of the glycocalyx are regulated on a molecular level, contribute to immune evasion and may be overcome t hr ou gh i mm un oe ng in eering must be resolved.. Here we report how cancer-associated mucins and their glycosylation contribute to the nanoscale material thickness of the glycocalyx and consequently modulate the functional interactions with cytotoxic immune cells.
Article Title: Mucins form a nanoscale material barrier against immune cell attack
Article Snippet: The unmodified human cDNA for GCNT1 was generated by custom gene synthesis (General Biosystems). .. The human HER2 cDNA was amplified by PCR from the plasmid, HER2 WT (Addgene #16257), with primers that added 5’ BamHI and 3’ BsrGI restriction sites: 5’-TAATCTAGACCCAAGCTTGGGGCAG -3’ and 5’-GCTGTCGACGAGCTCGGTACCAAGCTT-3’. cDNA for human LGALS3 was amplified by PCR from a pET21a expression vector (gift of C. Bertozzi) with primers that added 5’ BamHI and 3’ BsrGI restriction sites: 5’-TAAGCGGATCCGAATTCTATGGCAGACAATT-3’ and 5’-TGCTTTGTACATTATATCATGGTATATGAAGC-3’. cDNA for a previously reported HER2-specific CAR( ) – including a humanized FRP5 scFV, CD8a hinge, CD28 transmembrane domain, CD28 signaling domain, and CD3ζ signaling domain – was synthesized with a 2A peptide/mTagBFP2 expression reporter by custom gene synthesis (Twist Bioscience) (See Supplemental Table 1 for the complete sequence). .. A pLentiCRISPR V2 plasmid for knockout of GNE was created by inserting the oligos, 5’-CACCGACATCAAGTTCAAAGAACTC -3’ and 5’-AAACGAGTTCTTTGAACTTGATGTC-3’, into the BsmBI restriction site following standard protocols. ( ) Recombinant GFP nanobody cDNA (PDB #3OGO_E) was generated by custom gene synthesis (IDT) and cloned into the BamHI and HindIII restriction sites of pTP1112 (Addgene #104158).
Polymerase Chain Reaction:Article Title: Immunoengineering can overcome the glycocalyx armour of cancer cells.
Article Snippet: Cancer cell glycocalyx is a major line of defence against immune surveillance.. However, how specific physical properties of the glycocalyx are regulated on a molecular level, contribute to immune evasion and may be overcome t hr ou gh i mm un oe ng in eering must be resolved.. Here we report how cancer-associated mucins and their glycosylation contribute to the nanoscale material thickness of the glycocalyx and consequently modulate the functional interactions with cytotoxic immune cells.
Article Title: Mucins form a nanoscale material barrier against immune cell attack
Article Snippet: The unmodified human cDNA for GCNT1 was generated by custom gene synthesis (General Biosystems). .. The human HER2 cDNA was amplified by PCR from the plasmid, HER2 WT (Addgene #16257), with primers that added 5’ BamHI and 3’ BsrGI restriction sites: 5’-TAATCTAGACCCAAGCTTGGGGCAG -3’ and 5’-GCTGTCGACGAGCTCGGTACCAAGCTT-3’. cDNA for human LGALS3 was amplified by PCR from a pET21a expression vector (gift of C. Bertozzi) with primers that added 5’ BamHI and 3’ BsrGI restriction sites: 5’-TAAGCGGATCCGAATTCTATGGCAGACAATT-3’ and 5’-TGCTTTGTACATTATATCATGGTATATGAAGC-3’. cDNA for a previously reported HER2-specific CAR( ) – including a humanized FRP5 scFV, CD8a hinge, CD28 transmembrane domain, CD28 signaling domain, and CD3ζ signaling domain – was synthesized with a 2A peptide/mTagBFP2 expression reporter by custom gene synthesis (Twist Bioscience) (See Supplemental Table 1 for the complete sequence). .. A pLentiCRISPR V2 plasmid for knockout of GNE was created by inserting the oligos, 5’-CACCGACATCAAGTTCAAAGAACTC -3’ and 5’-AAACGAGTTCTTTGAACTTGATGTC-3’, into the BsmBI restriction site following standard protocols. ( ) Recombinant GFP nanobody cDNA (PDB #3OGO_E) was generated by custom gene synthesis (IDT) and cloned into the BamHI and HindIII restriction sites of pTP1112 (Addgene #104158).
Plasmid Preparation:Article Title: Immunoengineering can overcome the glycocalyx armour of cancer cells.
Article Snippet: Cancer cell glycocalyx is a major line of defence against immune surveillance.. However, how specific physical properties of the glycocalyx are regulated on a molecular level, contribute to immune evasion and may be overcome t hr ou gh i mm un oe ng in eering must be resolved.. Here we report how cancer-associated mucins and their glycosylation contribute to the nanoscale material thickness of the glycocalyx and consequently modulate the functional interactions with cytotoxic immune cells.
Article Title: Mucins form a nanoscale material barrier against immune cell attack
Article Snippet: The unmodified human cDNA for GCNT1 was generated by custom gene synthesis (General Biosystems). .. The human HER2 cDNA was amplified by PCR from the plasmid, HER2 WT (Addgene #16257), with primers that added 5’ BamHI and 3’ BsrGI restriction sites: 5’-TAATCTAGACCCAAGCTTGGGGCAG -3’ and 5’-GCTGTCGACGAGCTCGGTACCAAGCTT-3’. cDNA for human LGALS3 was amplified by PCR from a pET21a expression vector (gift of C. Bertozzi) with primers that added 5’ BamHI and 3’ BsrGI restriction sites: 5’-TAAGCGGATCCGAATTCTATGGCAGACAATT-3’ and 5’-TGCTTTGTACATTATATCATGGTATATGAAGC-3’. cDNA for a previously reported HER2-specific CAR( ) – including a humanized FRP5 scFV, CD8a hinge, CD28 transmembrane domain, CD28 signaling domain, and CD3ζ signaling domain – was synthesized with a 2A peptide/mTagBFP2 expression reporter by custom gene synthesis (Twist Bioscience) (See Supplemental Table 1 for the complete sequence). .. A pLentiCRISPR V2 plasmid for knockout of GNE was created by inserting the oligos, 5’-CACCGACATCAAGTTCAAAGAACTC -3’ and 5’-AAACGAGTTCTTTGAACTTGATGTC-3’, into the BsmBI restriction site following standard protocols. ( ) Recombinant GFP nanobody cDNA (PDB #3OGO_E) was generated by custom gene synthesis (IDT) and cloned into the BamHI and HindIII restriction sites of pTP1112 (Addgene #104158).
Expressing:Article Title: Mucins form a nanoscale material barrier against immune cell attack
Article Snippet: The unmodified human cDNA for GCNT1 was generated by custom gene synthesis (General Biosystems). .. The human HER2 cDNA was amplified by PCR from the plasmid, HER2 WT (Addgene #16257), with primers that added 5’ BamHI and 3’ BsrGI restriction sites: 5’-TAATCTAGACCCAAGCTTGGGGCAG -3’ and 5’-GCTGTCGACGAGCTCGGTACCAAGCTT-3’. cDNA for human LGALS3 was amplified by PCR from a pET21a expression vector (gift of C. Bertozzi) with primers that added 5’ BamHI and 3’ BsrGI restriction sites: 5’-TAAGCGGATCCGAATTCTATGGCAGACAATT-3’ and 5’-TGCTTTGTACATTATATCATGGTATATGAAGC-3’. cDNA for a previously reported HER2-specific CAR( ) – including a humanized FRP5 scFV, CD8a hinge, CD28 transmembrane domain, CD28 signaling domain, and CD3ζ signaling domain – was synthesized with a 2A peptide/mTagBFP2 expression reporter by custom gene synthesis (Twist Bioscience) (See Supplemental Table 1 for the complete sequence). .. A pLentiCRISPR V2 plasmid for knockout of GNE was created by inserting the oligos, 5’-CACCGACATCAAGTTCAAAGAACTC -3’ and 5’-AAACGAGTTCTTTGAACTTGATGTC-3’, into the BsmBI restriction site following standard protocols. ( ) Recombinant GFP nanobody cDNA (PDB #3OGO_E) was generated by custom gene synthesis (IDT) and cloned into the BamHI and HindIII restriction sites of pTP1112 (Addgene #104158).
Synthesized:Article Title: Mucins form a nanoscale material barrier against immune cell attack
Article Snippet: The unmodified human cDNA for GCNT1 was generated by custom gene synthesis (General Biosystems). .. The human HER2 cDNA was amplified by PCR from the plasmid, HER2 WT (Addgene #16257), with primers that added 5’ BamHI and 3’ BsrGI restriction sites: 5’-TAATCTAGACCCAAGCTTGGGGCAG -3’ and 5’-GCTGTCGACGAGCTCGGTACCAAGCTT-3’. cDNA for human LGALS3 was amplified by PCR from a pET21a expression vector (gift of C. Bertozzi) with primers that added 5’ BamHI and 3’ BsrGI restriction sites: 5’-TAAGCGGATCCGAATTCTATGGCAGACAATT-3’ and 5’-TGCTTTGTACATTATATCATGGTATATGAAGC-3’. cDNA for a previously reported HER2-specific CAR( ) – including a humanized FRP5 scFV, CD8a hinge, CD28 transmembrane domain, CD28 signaling domain, and CD3ζ signaling domain – was synthesized with a 2A peptide/mTagBFP2 expression reporter by custom gene synthesis (Twist Bioscience) (See Supplemental Table 1 for the complete sequence). .. A pLentiCRISPR V2 plasmid for knockout of GNE was created by inserting the oligos, 5’-CACCGACATCAAGTTCAAAGAACTC -3’ and 5’-AAACGAGTTCTTTGAACTTGATGTC-3’, into the BsmBI restriction site following standard protocols. ( ) Recombinant GFP nanobody cDNA (PDB #3OGO_E) was generated by custom gene synthesis (IDT) and cloned into the BamHI and HindIII restriction sites of pTP1112 (Addgene #104158).
Sequencing:Article Title: Mucins form a nanoscale material barrier against immune cell attack
Article Snippet: The unmodified human cDNA for GCNT1 was generated by custom gene synthesis (General Biosystems). .. The human HER2 cDNA was amplified by PCR from the plasmid, HER2 WT (Addgene #16257), with primers that added 5’ BamHI and 3’ BsrGI restriction sites: 5’-TAATCTAGACCCAAGCTTGGGGCAG -3’ and 5’-GCTGTCGACGAGCTCGGTACCAAGCTT-3’. cDNA for human LGALS3 was amplified by PCR from a pET21a expression vector (gift of C. Bertozzi) with primers that added 5’ BamHI and 3’ BsrGI restriction sites: 5’-TAAGCGGATCCGAATTCTATGGCAGACAATT-3’ and 5’-TGCTTTGTACATTATATCATGGTATATGAAGC-3’. cDNA for a previously reported HER2-specific CAR( ) – including a humanized FRP5 scFV, CD8a hinge, CD28 transmembrane domain, CD28 signaling domain, and CD3ζ signaling domain – was synthesized with a 2A peptide/mTagBFP2 expression reporter by custom gene synthesis (Twist Bioscience) (See Supplemental Table 1 for the complete sequence). .. A pLentiCRISPR V2 plasmid for knockout of GNE was created by inserting the oligos, 5’-CACCGACATCAAGTTCAAAGAACTC -3’ and 5’-AAACGAGTTCTTTGAACTTGATGTC-3’, into the BsmBI restriction site following standard protocols. ( ) Recombinant GFP nanobody cDNA (PDB #3OGO_E) was generated by custom gene synthesis (IDT) and cloned into the BamHI and HindIII restriction sites of pTP1112 (Addgene #104158).
|
Addgene inc is a verified supplier 